Pomegranate seed oil (PSO) has been used in the traditional medicine for the different kinds of pain. The less significant anti-nociceptive effect of LC than PSO and NCF shown in the present study could be related to that it has protective effect on C-fiber mitochondria rather than the A-fiber mitochondria which is evoked in PT neuropathy.īackground: Hyperalgesia and allodynia are abnormal sensory signs which are usually seen along neuropathic pain (NP) in patients on paclitaxel (PT) chemotherapy. The analgesic effect of LC had gained much interest in different forms of neuropathy conditions, and the significant anti-nociceptive effect of LC shown in the present study is in agreement with the findings of other clinical studies and experimental models of NP including chemotherapy-induced NP that confirmed the important role of LC in maintaining mitochondrial energy homeostasis and detoxification, nerve growth factor, and promoting peripheral nerve regeneration. The significant improvements in withdrawal latency obtained with NCF administration in PIPN compared to PTc group shown in this study indicate it possess neuroprotective and anti-nociceptive activity, which have also been demonstrated in different types of neurological conditions that elucidate its role in the structural neuroregeneration since nucleotides serve as a potential source of extracellular ATP and other purines that regulate the actions of many processes in the body including neurons. These results indicate that the nucleotides included in Nucleo CMP Forte ® are promising therapeutic molecules for the prevention of neuronal death in brain caused by focal ischemia, Parkinson's disease or other neurodegenerative pathologies. More interestingly, drug pre-treatment significantly reduced MPP + -and glutamate-induced cell death in SH-SY5Y cells and in rat cortical cells. Nucleo CMP Forte ® pre-treatment significantly increased the rate of cell division in SH-SY5Y cells, as well as the synthesis of triglycerides and phospholipids. Cell viability was measured at different times. We used the human dopaminergic cell line SH-SY5Y and a primary culture of rat cortical cells pre-treated with the drug for 24 hours and then exposed to MPP + or glutamate at a range of concentrations. We examined its neuroprotective effects on cell toxicity induced by glutamate excitotoxicity or by 1-methyl-4-phenyl-pyridinium (MPP +), an in vitro cell model of Parkinson's disease. Its effects on brain pathologies has received little attention.
It has been prescribed for peripheral nervous system disorders, such as lumbo-sciatalgia, diabetic or alcoholic polyneuropathy, or trigeminal neuralgia. Nucleo CMP Forte ® is a nucleotide-based drug consisting of cytidinemonophosphate, uridinemonophosphate, uridine-diphosphate and uridinetriphosphate.